GFP Mutants with Altered Mass Spectra Research Proposal - 1

GFP Mutants with Altered Mass Spectra - Research Proposal Example The green fluorescent protein (GFP), was discovered and isolated from the from the jellyfish Aequorea victoria (1). The native protein contains 238 amino acids which fold into six alpha helices and eleven beta strands forming a classical cylindrical beta sheet with anti-parallel strands. The GFP protein owes its fluorescence to the derived chromophore that is buried deep in the beta-barrel which protects interactions with the solvent. Due to its stable fluorescence, GFP has been used in biological studies as an important marker for processes like protein trafficking. Sequence data for GFP has led to the development of recombinant GFP proteins with modifications to increase the fluorescence intensity and longevity. There are many mutant GFPs with differences in absorption and emission spectra. There are enhanced GFP (EGFP) that has higher-intensity emission after blue-light excitation compared to wild-type GFP (4) (3). Another is GFPuv, a recombinant GFP with maximum emission spectra is 509nm, similar to that of wild-type GFP. GFPuv has a molecular weight of 29 kDa with three amino acid substitutions (Phe-99 to Ser, Met-153 to Thr, and Val-163 to Ala resulting in 18 times more brightness under ultra-violet (UV) light at 395nm. GFPuv is 18 times brighter than wild-type GFP. The large fluorescence of GFPuv making it suitable for experiments that require detection of changes in fluorescence. GFPuv expressed in highly expressed in E. coli is a soluble, fluorescent protein in contrast to wild-type GFP, which may be expressed in inclusion bodies as a nonfluorescent protein.

International financial management Essay Example | Topics and Well Written Essays - 1500 words - 1

International financial management - Essay Example Zeus Plc can apply for the direct loan from the host government in order to finance its project. The host country i.e. Malaysia must arrange for the venture capital in order to reduce the financial risk associated with Zeus Plc. Country risk was described by Levi (1990) as the type of risk that, because of a consequence of war, social or political events, or revolution; a firm might not be rewarded or paid for their exports. It applies to credit granted within trade and also to the foreign investment (Kosmidou, Doumpos and Zopounidis, 2010). However, it is also a part of financial risk. Scholars describe financial risk as a common term that is used for multiple risks that are close associated with and involves financial transaction (Frenkel et al, 2004; Siddaiah, 2010). In this regards, notion of debt and equity also becomes prevalent. The relation between debt and equity reflects the debt used for financing a company and equity of shareholders. In terms of the country risk ranking, UK is at 19th place as compared to 11th position in 2008. The score declines which means that in general the country risk of UK is 10 points less than the standard score designed for the AAA sovereigns (See Appendix 1 (Euromoney, 2012). As of now, its country risk rating is A2 (Globaledge, 2014). Three types of ventures have been discussed i.e. joint venture, franchising, and subsidiary. Joint venture is the type of strategic alliance of two different companies where both of them decide to act jointly, normally forming a two different legal entity, for a similar purpose (Uta, 2001; Van and Wachowicz, 2008). Lima (2008) stated a subsidiary means a unit which is actually controlled by a different entity (Epstein and Jermakowicz, 2010). Zeus Plc is assumed to be a large global mining company in the United Kingdom, which is deeming to extract minerals from the deposits which it owns at Tapir River in Malaysia. Country risk is

The effects to the white star line company because of the titanic Research Paper

The effects to the white star line company because of the titanic sinking - Research Paper Example The world has always fixed all attention on identifying the cause of the tragedy, while little effort has been shifted towards identifying the effect of the Titanic tragedy on the White Star Lines Company, the Liverpool-based company that owned the mega ship. The following report analyzes how the sinking of the Titanic impacted on the White Star Line company. The company prided itself with being the maker of the most luxurious ship at the time. The name of this wonder ship was Titanic. So much was the hype behind Titanic that it was commonly regarded as the unsinkable ship (Ziakas 109). By building the ship, the company had made set the benchmark for the trend in luxurious ships. The massive size of Titanic also increased the pride that came along with the ship since it was the biggest at the time. This intense over confidence in the success of the ship contributed majorly to the disaster, since adequate preventive measures were not put in place. The sinking of the ship had massive impact on the parent company, White Star Line, as illustrated in this report. The White Star Line refers to a ship building company which originally made traditional sailing ships that travelled from UK to Australia in the 1860s. The company majored on this route due to gold in Australia. This venture turned out to be very competitive hence it merged with other rival companies. The company merged with other companies such as The Black Ball and The Eagle Lines of ship (Butler 104). Under this partnership, the first White Star Line ship was launched in August 1870. The ship, known as Oceanic, was a break from the norm with its size, appearance and a host of other innovations. It was to be the world’s first true super liner and it gained popularity from all corners of the world. Later that year, the company launched three other identical ships, known as Atlantic, Republic and Baltic. In the following year, the company launched another ship, slightly larger than

Music and Childhood Development Literature review

Music and Childhood Development - Literature review Example At this age, they can use their sense organs to coordinate to actions although not in a systematic manner. Music, according to these researchers contributes to a child’s growth. Rhythm is an essential in childhood development as the child first hears the mother’s heartbeat at the prenatal environment (Campbell and Scott-Kassner, 156). Rhythm can be witnessed in the movements infants make with others or alone. According to Chau and Riforgiate’s research, listening to music has two impacts. One is what the children learn from listening, and the other is how listening to particular forms of music impact the child’s development outside music environment. Researches conducted show the ability of children to understand different sounds from instruments in different families and also different instruments within same instrument family (Chau and Riforgiate, 5). The research shows children between the ages of 3 to 7 years able to differentiate between different ins truments as per size, as well as pitch. Children between the ages of 3 to 4 years could differentiate various musical families but could not differentiate instruments in the same family. This shows how music affects childhood development at different stages. Another research article by Levinowitz argues that early childhood is the duration of quick change and development. The research argues that even the youngest infant can receive music and discriminate it according to differences in frequency, melody and stimuli. Music at this stage is as important to the child as babbling is, when the child tries to talk. Claudia Gold, another researcher, argues that music affects how the brain of a child works. She proves there is scientific evidence that music influences children... According to the above research articles and books, music has come out to have effect on the growth of children. The sensitive early years of children contribute much to their future behaviors. The researches have shown that children who get exposed to music at their early ages perform better in other fields than those that have no exposure on music. This is an evidence of the impacts that music has on childhood development. Music opens up a child’s mind in their early days, and it completely affects their lives in the future. This report makes a conclusion that a journal of neuroscience published a study that suggests that musical training before the age of seven affects how children think. This is a report, according to the Science Daily. The research shows that ages between six and eight years are sensitive such that their listening to music affects the development of the mind. The ability of a child to play a musical instrument comes from the ability of the mind to coordinate with the body. The effect of music in children’s minds is evident from early ages because it affects how children think, and the effect follows the children’s activities. Many successful musicians, according to Turner, come from families which practiced music together at home. These families have generational traditions in music. This contributes to how children grow. Sibal presents research conducted by The International Foundation of Music Research, which finds that babies are aware of music, and can respond to it and different sounds inside their mothers’ wombs.

Personal and professional growth statement with goals Research Paper

Personal and professional growth statement with goals - Research Paper Example Sub-consciously, learners teach more to their teachers than they can fathom. Another belief that I came to view as superstitious is the belief that education was designed solely for equipping an individual with intelligence. Unfortunately, this is the same thought that most people have and the truth of the matter is that this is a misguided point of view. One of the major objectives of education is to help a learner or a child realize the inborn capabilities and talents and the potential that they possess (Gardner 34). If articulated in the right manner, education will help the learner to be able to develop and nurture problem solving skills on a personal level. These are the two major realizations that have helped me grow as a person and as a future professional educator; the second being the most impactful. As discussed, the first realization that has had an impact on my way of thinking is the fact that learning is an interactive process in totality. As a classroom based educator, my number one objective should be to create a conducive classroom climate. A conducive classroom climate is one that encourages the participation of all the students in class. For example, group discussions is one of the ways that can promote a conducive classroom climate. ... School climate is based on the experiences that one gets from school life and reflects the norms, goals values, inter-personnel relationships, teaching, learning and leadership practices. As an elementary educator, my goal is to help create and develop a school climate that produces a holistic student (Hinman, 96). My second realization has helped me understand the importance of education in helping an individual to realize their in-born capabilities and talents. Through the theory of multiple intelligences, Howard Gardner explains that there are nine multiple intelligences that are present in humans (41) . These intelligences are in-built and the role of education is to nurture them to perfection for the attainment of their full potential. Linguistic intelligence is the first kind of intelligence that Gardner highlights. This is the ability of an individual to use language to express their thoughts. Most elementary students are very vibrant and proactive in speech. It is however not easy for a child to express their thoughts in a direct manner. If a child shows signs of linguistic intelligence, it is up to me as an elementary teacher to teach them how to nurture this talent and help them in development of their career. It is essential to help children grow in their linguistic intelligence because language is the most common and most accepted form of communication in the world. Another form of intelligence is logical thinking. It can also be referred to as mathematical thinking. Some children have the capabilities to understand the underlying principle of a casual system and can be able to manipulate numbers (Adsit, 67). When I become a professional educator, I will be teaching such kind of children how to use their capabilities to come up with inventions among other

Art extra credit Research Paper Example | Topics and Well Written Essays - 250 words

Art extra credit - Research Paper Example The major interest in the design results from the presentation of the real story in art. The actual story tells of the last supper comprising of a huge feast. The twelve disciples and Jesus had a great time and at the same time, Jesus informed the disciples of the traitor and they reacted harshly about the issue. The disciples spoke and showed their anger. The art reflects all the actions that took place in the last supper. However, the art shows how at that time people were united, loving, caring and honest. At the same time, a major lesson is derived from the art. Enemies are within the list of your friends as Judas was among the twelve disciples, but he betrayed Jesus. This lesson applies in real life, and one is greatly advised to take caution of his or her friends. The art designer Leonardo da Vinci did a great work. The actual date of the art is not well known, but the creation of the art was between 1494 and 1498. The dimensions of the art measure 460cm by 880cm. The art located at Santa Maria Delle Grazie in Milan. The designer regards the art as a huge lesson that every individual should take into consideration. Everyone should study the art as well as the actual baseline of the art for life

Vocabulary Parent Letter Essay Example for Free

Vocabulary Parent Letter Essay Vocabulary words and knowledge are critical in reading comprehension and vitally important that new and young readers develop a large ‘word bank’ and effective vocabulary learning strategies. That being said, we will have an emphasis on vocabulary words in our classroom this year. There are several effective vocabulary learning strategies that we will be using in the classroom, I encourage you to employ these strategies at home assisting in the reading comprehension and vocabulary development of your young reader. Effective Vocabulary Learning Strategies: †¢Pre-teaching Vocabulary Word – teaching your child unfamiliar words prior to the reading experience. An adult should preview the reading material determine unfamiliar words then define and discuss the words. This provides understanding of the word for the child as well as establishing connotation. It also gives the adult feedback on how well the child understands the word. †¢Repeated Exposure to Words – The more we are exposed to a word, the stronger our understanding become. A child needs to hear and use a word several times before it becomes part of their vocabulary. Provide multiple opportunities for the child to use a new word in written and spoken form. †¢Keyword Method – Like pre-teaching, the keyword method rather than having a child remember the definition, it encourages the adult to give a ‘word clue’ to help them understand the word. The idea behind the keyword method is to create an easy cognitive link to the word’s meaning that the reader can access efficiently during a reading experience. †¢Root Analysis – Many English words are derived from Latin or Greek roots. They contain a ‘core’ root or use prefixes or suffixes. Adults, when working with the student should focus on the commonly occurring roots, prefixes and suffixes. When a reader is able to break down unfamiliar words into their prefixes, suffixes and roots they can begin to determine their meanings. †¢Restructuring Reading Material – This strategy is particularly effective for struggling readers improving their vocabulary. Sometimes grade level materials contain too many unfamiliar words. Adults can restructure the material. Portions of the difficult words can be replaced with ‘easier’ synonyms to help the reader understand the overall text. Also, particularly challenging words can easily be ‘looked up’ while reading the text. Taking a few minutes to read with your child, using these strategies will truly aid their vocabulary development and reading comprehension for years to come. Let’s work together as a team to ensure that your child becomes an independent, lifelong learner.